Target Points in Trastuzumab Resistance

Epidermal growth factor (EGF) family of receptors is involved in cell growth and differentiation. The human EGF2 (HER2) lacks natural ligands, and correlation between HER2 levels and carcinogenesis makes the receptor an ideal candidate for targeted therapy in breast cancer. Trastuzumab is a humanized antibody applied against HER2-positive breast tumors in clinic. Metastatic tumors respond well to trastuzumab therapy for the first year, but development of antibody resistance helps the tumors to regrow allowing the disease to progress. Trastuzumab resistance is shaped via a range of intracellular signaling pathways that are interconnected and share in key effector molecules. Identification of a common node central to these resistance pathways could provide an ultimate solution for trastuzumab resistance in breast and other cancers

Precise motion descriptors extraction from stereoscopic footage using DaVinci DM6446

A novel approach to extract target motion descriptors in multi-camera video surveillance systems is presented. Using two static surveillance cameras with partially overlapped field of view (FOV), control points (unique points from each camera) are identified in regions of interest (ROI) from both cameras footage. The control points within the ROI are matched for correspondence and a meshed Euclidean distance based signature is computed. A depth map is estimated using disparity of each control pair and the ROI is graded into number of regions with the help of relative depth information of the control points. The graded regions of different depths will help calculate accurately the pace of the moving target and also its 3D location. The advantage of estimating a depth map for background static control points over depth map of the target itself is its accuracy and robustness to outliers. The performance of the algorithm is evaluated in the paper using several test sequences. Implementation issues of the algorithm onto the TI DaVinci DM6446 platform are considered in the paper

Modelling aggregation on the large scale and regularity on the small scale in spatial point pattern datasets

We consider a dependent thinning of a regular point process with the aim of obtaining aggregation on the large scale and regularity on the small scale in the resulting target point process of retained points. Various parametric models for the underlying processes are suggested and the properties of the target point process are studied. Simulation and inference procedures are discussed when a realization of the target point process is observed, depending on whether the thinned points are observed or not. The paper extends previous work by Dietrich Stoyan on interrupted point processes

Noncontact temperature pattern measuring device

Laser pyrometer techniques are utilized to accurately image a true temperature distribution on a given target without touching the target and without knowing the localized emissivity of the target. The pyrometer utilizes a very high definition laser beam and photodetector, both having a very narrow focus. The pyrometer is mounted in a mechanism designed to permit the pyrometer to be aimed and focused at precise localized points on the target surface. The pyrometer is swept over the surface area to be imaged, temperature measurements being taken at each point of focus

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth